Lazzaro Lab Publications

Click on titles for html links to journal websites or [pdf] to download article reprints.


Unckless, R.L. and B.P. Lazzaro. The potential for adaptive maintenance of diversity in insect antimicrobial peptides. Philosophical Transactions of the Royal Society, Biology, 371:20150291.[pdf]

Crawford, J.E., M.M. Riehle, K. Markianos, E. Bischoff, W.M. Guelbeogo, A. Gneme, N. Sagnon, K.D. Vernick, R. Nielsen, and B.P. Lazzaro. (2016) Evolution of GOUNDRY, a cryptic subgroup of Anopheles gambiae s.l., and its impact on susceptibility to Plasmodium infection. Molecular Ecology 25:1494-1510. [pdf]

Unckless, R.L.*, V.M. Howick*, and B.P. Lazzaro. (2016) Convergent balancing selection on an antimicrobial peptide in Drosophila. Current Biology 26:257-262 [pdf]
* denotes co-first authorship

Schwenke, R.A., B.P. Lazzaro, and M.F. Wolfner. (2016) The basis for immunity-reproduction tradeoffs in insects. Annual Review of Entomology 61:239-256. [pdf]


Crawford, J.E., M.M. Riehle, W.M. Guelbeogo, A. Gneme, N. Sagnon, K.D. Vernick, R. Nielsen, and B.P. Lazzaro. (2015) Reticulate speciation and barriers to introgression in the Anopheles gambiae species complex. Genome Biology and Evolution 7:3116-3131. [pdf]

Webster, C.L., F.M. Waldron, S. Robertson, D. Crowson, G. Ferrari, J.F. Quintana, J.M. Brouqui, E.H. Bayne, B. Longdon, A.H. Buck, B.P. Lazzaro, J. Akorli, P.R. Haddrill and D.J. Obbard. (2015) The discovery, distribution, and evolution of viruses associated with Drosophila melanogaster. PLoS Biology 13(7):e1002210. [pdf]

Lazzaro, B.P. (2015) Adenosine signaling and the energetic costs of induced immunity. PLoS Biology 13(4): e1002136. [pdf]
Commentary on Bajgar et al. 2015

Unckless, R.L., S.M. Rottschaefer and B.P. Lazzaro. (2015) The complex contributions of genetics and nutrition to immunity in Drosophila melanogaster. PLoS Genetics 11(3): e1005030. [pdf]

Unckless, R.L., S.M. Rottschaefer and B.P. Lazzaro. (2015) A genome-wide association study for nutritional indices in Drosophila. G3: Genes, Genomes, Genetics 5(3):417-425. [pdf]

Dobson, A.J., J.M Chaston, P.D. Newell, S.L. Ali, L. Donahue, D.R. Sannino, S. Westmiller, A. C.-N. Wong, A.G. Clark, B.P. Lazzaro and A.E. Douglas. (2015) Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis in Drosophila melanogaster. Nature Communications 6:6312. [pdf]

Rottschaefer, S.M, J.E. Crawford, M.M. Riehle, W.M. Guelbeogo, A. Gneme, N. Sagnon, K.D. Vernick and B.P. Lazzaro. (2015) Population genetics of Anopheles coluzzii immune pathways and genes. G3: Genes, Genomes, Genetics 5(3):329-339. [pdf]

Khalil, S., E. Jacobson, M.C. Chambers and B.P. Lazzaro. (2015) Systemic bacterial infection and immune defense phenotypes in Drosophila melanogaster. Journal of Visualized Experiments 99:e52613. [pdf]


Chambers, M.C., E. Jacobson, S. Khalil and B.P. Lazzaro (2014) Thorax injury lowers resistance to infection in Drosophila melanogaster. Infection and Immunity 82:4380-4389. [pdf]

Lazzaro, B.P. and D.S. Schneider (2014) The Genetics of Immunity Genetics 197:467-470; G3: Genes, Genomes, Genetics 4:943-945. [pdf]

Howick, V.M. and B.P. Lazzaro (2014) Genotype and diet shape resistance and tolerance across distinct phases of bacterial infection BMC Evolutioary Biology 14:56. [pdf]


Short, S.M. and B.P. Lazzaro (2013) Reproductive status alters transcriptomic response to infection in female Drosophila melanogaster G3: Genes, Genomes, Genetics 3:827-840. [pdf]

del Campo, M.L, R. Halitschke, S.M. Short, B.P. Lazzaro and A. Kessler. (2013) Dietary plant phenolic mediates tolerance to bacterial infection in Manduca sexta caterpillars. Entomologia Experimentalis et Applicata 146:321-331. [pdf]

Crawford, J.E., S.M. Rottschaefer, B. Coulibaly, M. Sacko, O. Niaré, M.M. Riehle, S.F. Traore, K.D. Vernick and B.P. Lazzaro (2013) No evidence for positive selection at two potential targets for malaria transmission-blocking vaccines in Anopheles gambiae s.s. Infection, Genetics, and Evolution 16:97-92 [pdf]


Crawford, J.E., E. Bischoff, T. Garnier, A. Gneme, K. Eiglmeier, I. Holm, M.M. Riehle, W.M. Guelbeogo, N. Sagnon, B.P. Lazzaro and K.D. Vernick (2012) Evidence for population-specific positive selection on immune genes of Anopheles gambiae. Genes, Genomes, Genetics 2:1505-1519. [pdf]

Galac, M.R. and B.P. Lazzaro (2012) Comparative genomics of bacteria in the genus Providencia isolated from wild Drosophila melanogaster. BMC Genomics 13:612. [pdf]

Rottschaefer, S.M. and B.P. Lazzaro (2012) No effect of Wolbachia on resistance to intracellular infection by pathogenic bacteria in Drosophila melanogaster. PLoS One 7:e40500. [pdf]

Short, S.M., M.F. Wolfner and B.P. Lazzaro (2012) Female Drosophila melanogaster suffer reduced defense against infection due to seminal fluid components. Journal of Insect Physiology 58:1192-1201. [pdf]

Crawford, J.E. and B.P. Lazzaro (2012) Assessing the accuracy and power of population genetic inference from low-pass next-generation sequencing data. Frontiers in Evolutionary and Population Genetics 3:66. [pdf]

Lazzaro, B.P. and A.G. Clark. (2012) "Rapid evolution of innate immune response genes." In Rapidly Evolving Genes and Genetic Systems, R.S. Singh, J. Xu and R.J. Kulathinal, eds. Oxford University Press, Oxford, UK. [request a pdf of the chapter]


McKean, K.A. and B.P. Lazzaro. (2011) "The costs of immunity and the evolution of immunological defense mechanisms." In Molecular Mechanisms of Life History Evolution , A. Heyland and T. Flatt, eds. Oxford University Press, Oxford, UK. [request a pdf of the chapter]

Lazzaro, B.P. and J. Rolff. (2011) Danger, microbes, and homeostasis. Science 332:43-4. [pdf]

Rottschaefer, S., M.M. Riehle, B. Coulibaly, M. Sacko, O. Niare, I. Morlais, S.F. Traore, K.D. Vernick and B.P. Lazzaro. (2011) Exceptional diversity, maintenance of polymorphism, and recent directional selection on the APL1 malaria resistance genes of Anopheles gambiae. PLoS Biology 9:e1000600. [pdf]

Galac, M. and B.P. Lazzaro. (2011) Comparative pathology of bacteria in the genus Providencia to a natural host, Drosophila melanogaster. Microbes and Infection13:673-683.[pdf]

Fellous, S. and B.P. Lazzaro. (2011) Potential for evolutionary coupling and decoupling of larval and adult immune gene expression. Molecular Ecology 20:1558-1567. [pdf]


Crawford, J.E., W.M. Guelbeogo, A. Sanou, A. Traore, K.D. Vernick, N. Sagnon and B.P. Lazzaro. (2010) De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology. PLoS One, 5:314202. [pdf]
gz archive of all annotated contigs in CSV format (10.7 Mb)
Single compressed FASTA file of all contigs (5.8 Mb)
Archive of all contigs in separate fasta files (6.3 Mb)
Python script automating iterative Velvet assembly approach used to assemble transcriptome

Short, S.M. and B.P. Lazzaro. (2010) Female and male genetic contributions to female post-mating susceptibility to infection in Drosophila melanogaster. Proceedings of the Royal Society, Biological Sciences, 277:3649-3657. [pdf]

Juneja, P. and B.P. Lazzaro. (2010) Haplotype structure and expression divergence at the Drosophila cellular immune gene eater. Molecular Biology and Evolution, 27:2284-2299.
[supplementary material]

Crawford, J. and B.P. Lazzaro (2010) The demographic histories of the molecular forms of Anopheles gambiae s.s. Molecular Biology and Evolution, 27:1739-1744. [pdf]

Fellous, S. and B.P. Lazzaro. (2010) Larval food quality affects adult (but not larval) immune gene expression independent of effects on general condition. Molecular Ecology, 19:1462-1468. [pdf]

Sackton, T.B, B.P. Lazzaro and A.G. Clark (2010) Genotype and gene expression associations with immune function in Drosophila PLoS Genetics, 6:e1000797. [pdf]


Juneja, P. and B.P. Lazzaro. (2009) "Population genetics of insect immune responses."ť In Insect Infection and Immunity, J. Rolff and S. Reynolds eds. [request a pdf of the chapter]

Juneja, P. and B.P. Lazzaro. (2009) Providencia sneebia sp. nov. and P. burhodogranareia sp. nov., novel species isolated from wild Drosophila melanogaster. International Journal of Systematic and Evolutionary Microbiology 59:1108-11. [pdf]

Hardstone, M.C., B.P. Lazzaro and J.G. Scott. (2009) The effect of three environmental conditions on the fitness of cytochrome P450 monooxygenase-mediated permethrin resistance in Culex pipiens quinquefasciatus. BMC Evolutionary Biology, 9:42. [pdf]

Lazzaro, B.P. and T.J. Little. (2009) Immunity in a variable world. Philosophical Transactions of the Royal Society, series B - Biology, 364:15-26. [pdf]


Riehle, M.M., J. Xu, B.P. Lazzaro, S.M. Rottschaefer, B. Coulibaly, M. Sacko, O. Niare, I. Morlais, S.F. Traore and K.D. Vernick. (2008) Anopheles gambiae APL1 is a family of variable LRR proteins required for Rel1-mediated protection from the malaria parasite, Plasmodium berghei. PLoS One 3:e3672.[pdf]

Schlenke, T.A. and B.P. Lazzaro (2008) Fruit flies like a (rotten) banana. Proceedings of the 49th annual GSA Drosophila Research Conference. Fly, 2:159-164. [pdf]

Lazzaro, B.P. (2008) Natural Selection on the Drosophila innate immune system. Current Opinion in Microbiology, 11:284-289. [pdf]

Lazzaro, B.P., H.A. Flores, J.G. Lorigan and C.P. Yourth. (2008) Genotype by environment interactions and adaptation to local temperature affect immunity and fecundity in Drosophila melanogaster. PLoS Pathogens 4:e1000025. [pdf]

McKean, K.A., C.P. Yourth, B.P. Lazzaro and A.G. Clark. (2008) The evolutionary costs of immunological maintenance and deployment. BMC Evolutionary Biology 8:76. [pdf]


Sackton, T.B., B.P. Lazzaro, T.A. Schlenke, J.D. Evans, D. Hultmark and A.G. Clark. (2007) Dynamic evolution of the innate immune system in Drosophila. Nature Genetics 39:1461-1468. [pdf]
[Supplemental Info (.zip archive)]

Drosophila 12 Genomes Consortium. (2007) Evolution of genes and genomes on the Drosophila phylogeny. Nature 450:203-18. [pdf]
[Supplemental Info (.zip archive)]


Lazzaro, B.P., T.B. Sackton and A.G. Clark. (2006) Genetic variation in Drosophila melanogaster resistance to infection: a comparison across bacteria. Genetics 174:1539-1554. [pdf]
Fig S1 (MS Excel) Fig S2 (Ms Excel)

The Honey Bee Genome Sequencing Consortium. (2006) Insights into social insects from the geonome of the honey bee Apis mellifera Nature 443:931-949. [pdf]
[Supplemental Info]

Lazzaro, B.P. and M.R. Galac. (2006) Dispatch. Disease Pathology: Wasting energy fighting infection. Current Biology 16:R964-R965. [pdf]

Rinkevich, F.D., L. Zhang, R.L. Hamm, S.G. Brady, B.P. Lazzaro and J.G. Scott. (2006) Allele frequencies of Vssc1 and CYP6D1 in house flies from the eastern United States. Insect Molecular Biology 15:157-167. [pdf]


Braverman, J.M., B.P. Lazzaro, M. Aguadé, and C.H. Langley. (2005) DNA sequence polymorphism and divergence at the erect wing and suppressor of sable loci of Drosophila melanogaster and D. simulans. Genetics 170:1153-65. [pdf]

Lazzaro, B.P. (2005) Elevated polymorphism and divergence in the class C scavenger receptors of Drosophila melanogaster and D. simulans. Genetics 169:2023-2034. [pdf]

Vernick, K., F. Oduol, B.P. Lazzaro, J. Glazebrook, J. Xu, M. Riehle and J. Li. (2005) Molecular Genetics of Mosquito Resistance to Malaria Parasites. Current Topics in Microbiology and Immunology 295:383-415. [pdf]

2004 and earlier

Lazzaro, B.P., B.K. Sceurman and A.G. Clark. (2004) The genetic basis of natural variation in D. melanogaster antibacterial immunity. Science 303:1873-1876. [pdf]
Fig S1 (MS Excel) Fig S2 (Ms Excel)

Lazzaro, B.P. and A.G. Clark. (2003) Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster. Molecular Biology and Evolution 20:914-923. [pdf]

Lazzaro, B.P., B.K. Sceurman, S.L. Carney and A.G. Clark. (2002) fRFLP and fAFLP: Medium throughput genotyping by post-labeling restriction digestions. BioTechniques 33:539-543. [pdf]

Lazzaro, B. P. and A.G. Clark. (2001) Evidence for recurrent paralogous gene conversion and exceptional allelic divergence in the Attacin genes of Drosophila melanogaster. Genetics 159:659-671. [pdf]
Supplemental Table S1 Supplemental Table S2

Langley, C.H., B.P. Lazzaro, W. Phillips, E. Heikkinen, and J. Braverman. (2000) Linkage disequilibria and the site frequency spectra in the su(s) and su(wa) regions of the Drosophila melanogaster X chromosome. Genetics 156:1837-1852 [pdf]

Carvalho, A.B, B.P. Lazzaro, and A.G. Clark. (2000) Y-chromosomal fertility factors kl-2 and kl-3 of D. melanogaster encode dynein heavy chain polypeptides. Proceedings of the National Academy of Sciences USA 97:13239-13244 [pdf]

Savolainen, O., C.H. Langley, B.P. Lazzaro, and H. Fréville. (2000) Contrasting patterns of nucleotide polymorphism at the Alcohol Dehydrogenase locus in the outcrossing Arabidopsis lyrata and the selfing Arabidopsis thaliana. Molecular Biology and Evolution 17:645-2000 [pdf]